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Cell debris was removed by centrifugation at 4,400 g at 4 C for 30 min, and the clarified cell lysate was directly used for biocatalytic reactions

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2021 Feb 2;25(1):76-80

Glob Cardiol Sci Pract

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[192] but under altered premises, Australian elderly (6084 y/o), supposedly representing a population group with low incidences of vitamin D deficiency, consumed 60,000 IU cholecalciferol on a monthly basis for five years, but no significant effects on hospitalization due to infection, despite a decline in the number of extended hospitalizations (over 6 d), could be observed, suggesting that general supplementation in people with adequate vitamin D serum concentrations exert only little effects, whereas its role in infectious disease is reinforced [194]