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Fiber-rich diets add to these advantages by supplying fermentable substrates that boost SCFA generation, increase regulatory T-cell differentiation, modify microglial maturation, and improve BBB function

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Cox proportional hazards models were used to estimate HRs

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Lysis was achieved through five to six iterative freeze-thaw cycles: frozen samples were thawed at 23 C in a thermomixer (Thermomixer Comfort, Eppendorf) until 60-80% liquefied, then held on ice until fully thawed, before being rapidly refrozen in liquid nitrogen

That familiar morning stiffness turned into persistent tenderness that forced me to cut training volume by half