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For LC-MS analysis, samples were thawed on ice-water bath, then 40 L of whole blood was injected into 450 L cold extraction solvent comprising of mixture of Acetonitrile:Methanol:Milli-Q water in ratio 40:40:20 according to protocol described in Presterud et al 35

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Purity is determined using High-Performance Liquid Chromatography (HPLC), which separates the target peptide from any potential impurities

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Cell 95, 927937, S0092-8674(00)81717-1 (1998)

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