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Clin Endocrinol (2009) 71(4):5006

When GHK binds to copper (Cu) ions, it forms a biologically active copper-peptide complex commonly investigated for its role in cellular communication and physiological-maintenance mechanisms

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Uncleaved protein, cleaved hexa-histidine tag, and 3C protease-GST tag were removed by passing the dialysed sample through a Glutathione agarose and a Ni-NTA column