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Subsequent restriction digest of PCR fragments with ApaI resulted in 141 bp and 516 bp fragments when positive for the F973 modification, whereas nonmodified WT DNA did not contain the ApaI site and thus still showed a 658 bp band upon ApaI digestion

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Furthermore, we used a commercial glycolysis antibody panel to detect Pfkp, Pyruvate kinase M1 and M2 (Pkm1, Pkm2), Hexokinase 1, Hexokinase 2 and Pyruvate dehydrogenase 1 subunit (Pdh) by immunoblot analysis

Vitamin B12 is essential for red blood cell formation, neurological function, and DNA synthesis